stem cell factor (Thermo Fisher)
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stem cell factor
Stem Cell Factor, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/stem+cell+factor/Stem-Cell+Factor%2Fc-Kit+Inhibitor/pmc13052131-45-48-52
Average 94 stars, based on 1 article reviews
Stem Cell Factor, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/stem+cell+factor/Stem-Cell+Factor%2Fc-Kit+Inhibitor/pmc13052131-45-48-52
Average 94 stars, based on 1 article reviews
stem cell factor - by Bioz Stars,
2026-10
94/100 stars
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Real-time Polymerase Chain Reaction:Article Title: Implications of stress-induced gene expression for hematopoietic stem cell aging studies. Article Snippet: .. Quantitative PCR with reverse transcription cKIT-enriched BM cells were isolated from young (2 months) and aging (20 months) C57BL/6 male mice and incubated at 37 °C for up to 16 h in DMEM High Glucose (Gibco) supplemented with 10% FCS, 0.1 mM 2-mercaptoethanol (Invitrogen), 1× penicillin-streptomycin-glutamine (Invitrogen), 50 ng ml−1 Reverse Transcription:Article Title: Implications of stress-induced gene expression for hematopoietic stem cell aging studies. Article Snippet: .. Quantitative PCR with reverse transcription cKIT-enriched BM cells were isolated from young (2 months) and aging (20 months) C57BL/6 male mice and incubated at 37 °C for up to 16 h in DMEM High Glucose (Gibco) supplemented with 10% FCS, 0.1 mM 2-mercaptoethanol (Invitrogen), 1× penicillin-streptomycin-glutamine (Invitrogen), 50 ng ml−1 Isolation:Article Title: Implications of stress-induced gene expression for hematopoietic stem cell aging studies. Article Snippet: .. Quantitative PCR with reverse transcription cKIT-enriched BM cells were isolated from young (2 months) and aging (20 months) C57BL/6 male mice and incubated at 37 °C for up to 16 h in DMEM High Glucose (Gibco) supplemented with 10% FCS, 0.1 mM 2-mercaptoethanol (Invitrogen), 1× penicillin-streptomycin-glutamine (Invitrogen), 50 ng ml−1 Article Title: Mast cells promote choroidal neovascularization in a model of age-related macular degeneration Article Snippet: .. Cells were isolated by centrifugation (500 g for 5 min) and resuspended in Roswell Park Memorial Institute (RPMI) 1640 medium (A1049101, Thermo Fisher) with glutamine, interleukin (IL)-3 (10 ng/mL), Incubation:Article Title: Implications of stress-induced gene expression for hematopoietic stem cell aging studies. Article Snippet: .. Quantitative PCR with reverse transcription cKIT-enriched BM cells were isolated from young (2 months) and aging (20 months) C57BL/6 male mice and incubated at 37 °C for up to 16 h in DMEM High Glucose (Gibco) supplemented with 10% FCS, 0.1 mM 2-mercaptoethanol (Invitrogen), 1× penicillin-streptomycin-glutamine (Invitrogen), 50 ng ml−1 Construct:Article Title: Genetic ablation of adhesion ligands mitigates rejection of allogeneic cellular immunotherapies. Article Snippet: IL15RF was constructed as previously described.43 Transfections were performed using a Neon Transfection System 100 ml Kit (Thermo Fisher) following the manufacturer’s protocol. iPSC were differentiated into hematopoietic progenitors and CD34+ cells using established methods.44,45 In brief, iPSC-derived CD34+ cells were transferred to NK cell differentiation B0 media (2:1 mixture of Dulbecco modified Eagle media and Ham F12 media supplemented with 2 mM L-glutamine, 100 U/mL penicillin, and 100 mg/mL streptomycin, 25 mM b-mercaptoethanol, 10% [V/V] human AB serum, 5 ng/ml sodium selenite, 50 uM ethanolamine, 20 mg/ml ascorbic acid [all ThermoFisher], 5 ng/mL IL-3 [first week only; ThermoFisher], 30 ng/mL stem cell factor [SCF ThermoFisher], 20 ng/mL IL-15 [RnD], and 10 ng/mL Fml-like tyrosine kinase 3 ligand [FLT3L; ThermoFisher]). .. IL15RF was constructed as previously described.43 Transfections were performed using a Neon Transfection System 100 ml Kit (Thermo Fisher) following the manufacturer’s protocol. iPSC were differentiated into hematopoietic progenitors and CD34+ cells using established methods.44,45 In brief, iPSC-derived CD34+ cells were transferred to NK cell differentiation B0 media (2:1 mixture of Dulbecco modified Eagle media and Ham F12 media supplemented with 2 mM L-glutamine, 100 U/mL penicillin, and 100 mg/mL streptomycin, 25 mM b-mercaptoethanol, 10% [V/V] human AB serum, 5 ng/ml sodium selenite, 50 uM ethanolamine, 20 mg/ml ascorbic acid [all ThermoFisher], 5 ng/mL IL-3 [first week only; ThermoFisher], 30 ng/mL Transfection:Article Title: Genetic ablation of adhesion ligands mitigates rejection of allogeneic cellular immunotherapies. Article Snippet: IL15RF was constructed as previously described.43 Transfections were performed using a Neon Transfection System 100 ml Kit (Thermo Fisher) following the manufacturer’s protocol. iPSC were differentiated into hematopoietic progenitors and CD34+ cells using established methods.44,45 In brief, iPSC-derived CD34+ cells were transferred to NK cell differentiation B0 media (2:1 mixture of Dulbecco modified Eagle media and Ham F12 media supplemented with 2 mM L-glutamine, 100 U/mL penicillin, and 100 mg/mL streptomycin, 25 mM b-mercaptoethanol, 10% [V/V] human AB serum, 5 ng/ml sodium selenite, 50 uM ethanolamine, 20 mg/ml ascorbic acid [all ThermoFisher], 5 ng/mL IL-3 [first week only; ThermoFisher], 30 ng/mL stem cell factor [SCF ThermoFisher], 20 ng/mL IL-15 [RnD], and 10 ng/mL Fml-like tyrosine kinase 3 ligand [FLT3L; ThermoFisher]). .. IL15RF was constructed as previously described.43 Transfections were performed using a Neon Transfection System 100 ml Kit (Thermo Fisher) following the manufacturer’s protocol. iPSC were differentiated into hematopoietic progenitors and CD34+ cells using established methods.44,45 In brief, iPSC-derived CD34+ cells were transferred to NK cell differentiation B0 media (2:1 mixture of Dulbecco modified Eagle media and Ham F12 media supplemented with 2 mM L-glutamine, 100 U/mL penicillin, and 100 mg/mL streptomycin, 25 mM b-mercaptoethanol, 10% [V/V] human AB serum, 5 ng/ml sodium selenite, 50 uM ethanolamine, 20 mg/ml ascorbic acid [all ThermoFisher], 5 ng/mL IL-3 [first week only; ThermoFisher], 30 ng/mL Cell Differentiation:Article Title: Genetic ablation of adhesion ligands mitigates rejection of allogeneic cellular immunotherapies. Article Snippet: IL15RF was constructed as previously described.43 Transfections were performed using a Neon Transfection System 100 ml Kit (Thermo Fisher) following the manufacturer’s protocol. iPSC were differentiated into hematopoietic progenitors and CD34+ cells using established methods.44,45 In brief, iPSC-derived CD34+ cells were transferred to NK cell differentiation B0 media (2:1 mixture of Dulbecco modified Eagle media and Ham F12 media supplemented with 2 mM L-glutamine, 100 U/mL penicillin, and 100 mg/mL streptomycin, 25 mM b-mercaptoethanol, 10% [V/V] human AB serum, 5 ng/ml sodium selenite, 50 uM ethanolamine, 20 mg/ml ascorbic acid [all ThermoFisher], 5 ng/mL IL-3 [first week only; ThermoFisher], 30 ng/mL stem cell factor [SCF ThermoFisher], 20 ng/mL IL-15 [RnD], and 10 ng/mL Fml-like tyrosine kinase 3 ligand [FLT3L; ThermoFisher]). .. IL15RF was constructed as previously described.43 Transfections were performed using a Neon Transfection System 100 ml Kit (Thermo Fisher) following the manufacturer’s protocol. iPSC were differentiated into hematopoietic progenitors and CD34+ cells using established methods.44,45 In brief, iPSC-derived CD34+ cells were transferred to NK cell differentiation B0 media (2:1 mixture of Dulbecco modified Eagle media and Ham F12 media supplemented with 2 mM L-glutamine, 100 U/mL penicillin, and 100 mg/mL streptomycin, 25 mM b-mercaptoethanol, 10% [V/V] human AB serum, 5 ng/ml sodium selenite, 50 uM ethanolamine, 20 mg/ml ascorbic acid [all ThermoFisher], 5 ng/mL IL-3 [first week only; ThermoFisher], 30 ng/mL Modification:Article Title: Genetic ablation of adhesion ligands mitigates rejection of allogeneic cellular immunotherapies. Article Snippet: IL15RF was constructed as previously described.43 Transfections were performed using a Neon Transfection System 100 ml Kit (Thermo Fisher) following the manufacturer’s protocol. iPSC were differentiated into hematopoietic progenitors and CD34+ cells using established methods.44,45 In brief, iPSC-derived CD34+ cells were transferred to NK cell differentiation B0 media (2:1 mixture of Dulbecco modified Eagle media and Ham F12 media supplemented with 2 mM L-glutamine, 100 U/mL penicillin, and 100 mg/mL streptomycin, 25 mM b-mercaptoethanol, 10% [V/V] human AB serum, 5 ng/ml sodium selenite, 50 uM ethanolamine, 20 mg/ml ascorbic acid [all ThermoFisher], 5 ng/mL IL-3 [first week only; ThermoFisher], 30 ng/mL stem cell factor [SCF ThermoFisher], 20 ng/mL IL-15 [RnD], and 10 ng/mL Fml-like tyrosine kinase 3 ligand [FLT3L; ThermoFisher]). .. IL15RF was constructed as previously described.43 Transfections were performed using a Neon Transfection System 100 ml Kit (Thermo Fisher) following the manufacturer’s protocol. iPSC were differentiated into hematopoietic progenitors and CD34+ cells using established methods.44,45 In brief, iPSC-derived CD34+ cells were transferred to NK cell differentiation B0 media (2:1 mixture of Dulbecco modified Eagle media and Ham F12 media supplemented with 2 mM L-glutamine, 100 U/mL penicillin, and 100 mg/mL streptomycin, 25 mM b-mercaptoethanol, 10% [V/V] human AB serum, 5 ng/ml sodium selenite, 50 uM ethanolamine, 20 mg/ml ascorbic acid [all ThermoFisher], 5 ng/mL IL-3 [first week only; ThermoFisher], 30 ng/mL Derivative Assay:Article Title: Inflammatory Mediators Both Directly and Indirectly Promote Microglial Proliferation. Article Snippet: .. We tested the following factors: nerve growth factor β (βNGF; Gibco 450–34), brain derived neurotrophic factor (BDNF; Gibco 450–02), colony stimulating factor- 3 (CSF3; Gibco AF- 250- 05), fractalkine (CX3CL1; Gibco 300–31), interferon- ɣ (IFNɣ; ThermoFisher Scientific BMS326), insulin- like growth factor 1 (IGF1; Gibco 250–19), interleukin- 17A (IL17A; Gibco 210- 17), interleukin- 1ɑ (IL1ɑ; R&D Systems 400- ML- 005), interleukin- 1β (IL1β; R&D Systems 401- ML- 005), interleukin- 33 (IL33; Gibco 210–33), interleukin- 4 (IL4; Gibco 214–14), interleukin- 5 (IL5; Gibco 215–15), lysophosphatidic acid (LPA; Sigma Aldrich 857128P), neurotensin (NTS; R&D Systems 1909), neuregulin- 1 (NRG1; Gibco 100–03), pleiotrophin (PTN; R&D Systems 6580- PL), Infection:Article Title: Generation of induced pluripotent stem cells from an HLA-B27 positive ankylosing spondylitis patient with syndesmophyte formation. Article Snippet: Human leukocyte antigen (HLA)-B27 is the genetic marker for ankylosing spondylitis (AS).. Here, we generated induced pluripotent stem cells (iPSCs) from peripheral blood mononuclear cells of a male AS patient carrying HLA-B27 with syndesmophyte formation by using the Sendai-virus delivery system.. The resulting iPSCs had a normal karyotype, expressed pluripotent markers, and could differentiate into three germ layers. Cell Culture:Article Title: Generation of induced pluripotent stem cells from an HLA-B27 positive ankylosing spondylitis patient with syndesmophyte formation. Article Snippet: Human leukocyte antigen (HLA)-B27 is the genetic marker for ankylosing spondylitis (AS).. Here, we generated induced pluripotent stem cells (iPSCs) from peripheral blood mononuclear cells of a male AS patient carrying HLA-B27 with syndesmophyte formation by using the Sendai-virus delivery system.. The resulting iPSCs had a normal karyotype, expressed pluripotent markers, and could differentiate into three germ layers. Centrifugation:Article Title: Mast cells promote choroidal neovascularization in a model of age-related macular degeneration Article Snippet: .. Cells were isolated by centrifugation (500 g for 5 min) and resuspended in Roswell Park Memorial Institute (RPMI) 1640 medium (A1049101, Thermo Fisher) with glutamine, interleukin (IL)-3 (10 ng/mL), other:Article Title: Targeted genetic and small molecule disruption of N-Ras CaaX cleavage alters its localization and oncogenic potential. Article Snippet: HEK-293 and HEK-293 T cells were routinely cultured using tissue culture 10 cm dishes or 250 mL vented |